The disc technique for determining sensitivity to the antibiotics.
نویسندگان
چکیده
The range of antibiotics available for therapy is extending steadily, and in consequence there is an increasing demand on the services of the laboratory. Chloramphenicol has now been added to the free list, aureomycin is being used for certain prescribed conditions, and terramycin is generally available in the U.S.A. This form of therapy should be subjected to bacteriological control, and it is therefore important to have a simple and reliable method of determining sensitivity. Various methods are used for these tests, and the serial dilution and the disc techniques are probably the most popular. The serial -dilution method is reliable, but it is time-consuming and consequently is of limited value as a routine test. The disc technique is now widely used for routinepurposes, as a quick result can be obtained. This is a simple adaptation of the original cylinder method, in which, it should be appreciated, the assays were made at least in duplicate under carefully controlled conditions. The demand for quick results has produced a variety of disc methods, some of which have little, apart from simplicity, to offer. It should, however, be accepted that, unless a consistent standard technique is -employed, the results may be very misleading. This is a matter of considerable importance if the tests are used for therapeutic control. There are many sources of error in the disc technique, and irregular results are not uncommon. The importance of the depth of agar in the assay plates has been discussed by Hayes (1945). Some other factors are now considered.
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عنوان ژورنال:
- Journal of clinical pathology
دوره 4 3 شماره
صفحات -
تاریخ انتشار 1951